10:00a – 9:00p
Ph Meeting (10:30-1:00p)
- More numbers
- simple fig for on rate / off rate model descrimination
- need more bio-info trends
- Psc knockdown
Mentoring
- Discussing Random Walk simulation algorithms
- explaining existing model code
- presented stripped down model code for further exploration
project 2 mentoring
- discuss gene annotations
- need to get gene list analyzed.
RNA quantification
- See Manual
- excitation maxima at 500, emission maxima 525. Must be narrow peaks, we tried excite at 450, scan absorption from 500+, and excite at 500 scan from 550+.
STORM4 troubleshooting
- TO get the AOTF to connect: Turn off computer. Turn off AOTF. Turn on AOTF. Turn on Computer.
- Current Quadview cube with notch filter is in POSITION 6 (as best as I can tell).
- This is different than what the key on the wall suggests (which would be 3) and different the setting I found in Colenso and Yari’s settings files (position 1).
- Position 3 gives horrible
- Non-Gaussian beamshape creates really weird diffraction pattern when aligning back-optics.
- short treatment with 561 beads, very few stuck.
- lamina stains look great.