Sunday 09/10/13

9:00a – 1:30p, 5:30 – 11:00p

Goals

  • Continue analysis of MiSeq Results (in process)
  • Work on modeling figures for Ph Project
  • Finish editing Banff Workshop report and send around for final review prior to submission. (tomorrow?)
  • Double check requirements for report submission. (tomorrow?)
  • Flip Fly Stocks! (tomorrow?)

Deep Sequencing Data Processing

  • savepaths got screwed up. Data saved with wrong name in wrong place and overwritten.
  • Attempting to fix this issue with some new general codes
  • Either c05 got contaminated with some of the pools prior to adding the adapters or these small libs have difficulty amplifying. Not sure if this is actually c05
  • Found at least 1 sequence with Black region internal sequence according to mapping label but c05 primer sequence.

General Coding

  • writing modified version of save to
    1. Avoid overwriting data without warning
    2. Let the user know where the file is actually being saved
    3. Handle conflicting names by prompting the user to overwrite or by automatically increasing the filenumber
  • Tried to combine this into save and saveas. Not so easy to use save in a new function because we have to pass it all of the variables.
  • I suppose one could save to scratch and load from scratch. — not really because the original save to scratch needs to happen before the function call.

OligoPaints project organization

  • Renamed ChromatinColors folder as Library1
  • cleaned up OligoPaintProbes folder (removed project2 folder which should be in project2, consolidated primer stuff).

Project 2 experiment prep

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