10:00a – 7:30p, 10:15p – 12:00a
Ph Project
Coding
- Cleaning up Ph simulation codes
- moving more stuff to functions
- sped up pivotMove
- removed loop in plotting function
- Most computation time now taken up by Mex Function call in pdist.
- see PRC1model_GeneralForm for the current fastest and cleanest polymer simulations.
Data analysis revisited
- clusters are 18 21×21 nm boxes ~ 8,000 nm^2 (80×80 nm). of order ~100 localizations
- Developed more streamlined cluster analysis routine. Still really need more data for total Ph in the different cell backgrounds. This antibody must not be too keen in vivo since the PhM flag expression is much stronger than what we see with Ph antibody in PhM cells.
Chromatin Analysis
- Analyze new D11 data (11/2). Spot finding not yet run. Re-running on Cajal in batches of 6 each. Hopefully this completes before scheduled reboot tomorrow.
- E10rna largely out of focus after image 1. Odd, since this was on STORM4
- E10dna pretty backgroundy. high variance in dot size.