9:50a – 5:30p, 10:00p – 12:15a
Goals
- file reimbursement paperwork for post-doc visit
- check to Colenso for ski trip
- help Sebastian design probes
chromatin project
- Run probe design for new chromatin regions selected to test off-diagonal HiC contacts
- Too many probes now, need to prune list
- current files only kept chromosome locus, want to look up and match the region description.
- rerunning with 76 76 secondary structure and cross-hybe cutoffs. Running on Cajal.
Ph project
- issues with cell variation — see notes
- setup imaging of PhM
Collaborations
- get probe sequences generated for SD
- discuss flow chamber issues with project 2 team.
- I recommend adding slides to the chamber — the solid glass adds a lot more structural support.
- Will look into controlling flow with syringe pumps — constant volume control will be good for mixed viscosity buffers.