9:30a – 12:45p
Lab organization
- measure laser powers on STORM4
- STORM users meeting — see summary sent in email.
Ph project
Paper to do
- We need to make the sup. fig1. showing no pri-antibody controls.
- added the flag in S2 cell images
- don’t have all the no primaries with DAPI, need to repeat
- Color of both images in Fig1 should be same. May be we should just keep the color of anti-FLAG as well as anti-PH staining as red.
- will make everything red in future
- I still need to put number at couple of places for chip-seq data.
- Ajaz will handle
- Need to fix the ticks on y-axis in Fig2.
- will do in future
- Black lines of bars in some graphs of cluster diameter are not there, can you check that.
- fixed this in Fig 1.
- will check other graphs in future.
- Review latest draft of text
- done. Fully revised all sections / methods / captions. Sent to Ajaz.
- get better PcG-body images of Ph in S2 cells.
- done. some cells a little bit backgroundy in the cytoplasm but the nuclear enrichment is clear.
- some cells have body like structures
Data analysis
- Analyzing on Cajal:
- F05-F06 two color data 750 chn
- F05-F06 two color data 647 chn
- F06-F05 two color data 750 chn
- F06-F05 two color data 647 chn
- S2 PH-STORM FISH
- PH-WT FLAG STORM FISH
- running at 80% CPU on Cajal no stalling :).
Chromatin project discussions
- Discussion with Pallav about teaming up on chromatin
- Write to JK about yeast chromatin topology / interpreting the yeast HiC papers.