10:20a – 11:30p
PH project
Simulations / model description
- Improved behavior from PH simulation
\Data\2014-03-17_PhSim\PhCell_CappingModel4
Main Figures
- wrote new scripts to graph the data in figures 1-3 quickly. Useful for changing binning intervals, fonts / colors / sizes etc easily.
- updated figures 1-3 to match new layout requests, fix match true-black, make scalebars equal width, match fontsizes, match panel spacing, correct cropping etc.
- sent new figures to Ajaz.
- BK currently reviewing manuscript.
Oligo-Secondaries
- working on figure
- wrote caption for current figure draft
- emailed draft and caption to Ting and Brian
Computer upgrades
- discussed windows server upgrades with CDW rep
- Ordered windows server updates
- need quote for server (can get remote desktop licenses through HHMI CDW portal).
- requested new quote with HHMI as bill to.
- configured remote desktop timeouts on cajal: http://technet.microsoft.com/en-us/library/cc754272.aspx
- disconnected state: 5 days until termination
- idle state: 2 days until disconnect
Chromatin Project
- set-up PCRs for probe making
- PCRs work (old get not so good and a bit overloaded). Some high MW product.
- also most samples mostly bubble band — expect 122 bp. Some samples have some low MW product, I wonder if this is mispriming? Should sequence this library too (MiSeq kit on the way).
- ran gel cleanup.
- set up T7 reactions to run O/N with RNasin inhibitor. Run started ~10:00p.
Data Analysis
- running bead-data compression for recently split multi-color data.
- Attempt resuming F12F11 data. Still missing 647 data for image 2. Now analyzing.