Thursday 04/17/14

STORM

• prism2 lost focus just after midnight (~minutes after I checked it and went home)
• prism2 now upgraded to STORM1. Jeff looking into peizo replacement. (How about a piezo swap with STORM4 dual-objective?)
• continue imaging on STORM2 sample E07

Staining

• forgot to check oven calendar, need to wait until 2pm to wash out probes.
• washing out probes on 6 new samples (3 dual-color lib2 samples and L3 E8 to E10 single color samples)
• Jeff recommends trying D2O imaging for low quantum yield dyes (e.g. A750).

Modeling

• test: expected polymer mixing.