9:00a – 8:35p
Project 2
- see post.
Chromatin project
- still imaging G08G06
- sometime rather abruptly between 9a and 11a the 750 dyes completely stopped switching off.
Microscope stuff
- Installing and aligning dual-view on STORM3
- figured out how to exchange dichroic filter cubes
- don’t have a proper cy3-cy5 dichroic
- this dual view is beat-to-heck, the adjuster knobs are all crazy loose and have very little purchase.
- ordered new cy3-cy5 cube from Semrock. Won’t pass 561 laser well, but should work excellently with 532.
STORM4
- imaging Lib3 E04E05 jointly stained sample.
- TIRF computer controller not working. Tuned by hand.
- laser alignment looks much better, bright, nice flat field. Thanks Jeff & Hazen!