9:30a – 5:00p, 10:30p – 12:00a
Goals
- Finish stains
- 2-clr STORM imaging En TAD regions
- Group meeting prep.
- Communication
- email Graham
- email Steph dates
Lab Meeting
- (laptop battery died, no e-notes).
- 750 with adaptive optics?
Probe making
- RT-PCRs didn’t work. (maybe screwed up primers)
- Repeat with B01-B08. These did work. Started amplifying around cycle 22. See PCR curves. 45 cycles all saturated before cycle 40.
STORM
- imaged bead fields
- imaging F03-p1 F04-p3 lib 3 double color with MEA. Looks pretty good, plenty of switching.