8:45a – 12:45a
9:00a-11:15a, lab volleyball practice
Probe synthesis
- B02-B09 3ul P1-A405, 10 uL RNA,
- plus 5 uL RT buffer, 2 uL dNTP, 1.5 uL ddH2O, 1.5 uL inhibitor, .2 ul RT
- Master = 27 uL P1-A405 + 45 uL RT buffer + 18 uL dNTP + 13.5 ddH2O + 13.5 uL inhibitor + 2 uL RT
- B02-B09 3uL P1-A647, 10 uL RNA,
- 5 uL RT buffer, 2 uL dNTP, 1.5 uL ddH2O, 1.5 uL inhibitor, .2 ul RT
- Master = 27 uL P1-A647 + 45 uL RT buffer + 18 uL dNTP + 13.5 ddH2O + 13.5 uL inhibitor + 2 uL RT
- F03-F11 4uL P1-A405 15 uL RNA,
- plus 6 uL RT buffer, 3 uL dNTP, 2 uL inhibitor, .2 uL RT
- Master = 36 uL P1-A405 + 54 uL RT buffer + 27 uL dNTP + 18 uL RNAsin + 3 uL RT
- F03-F11 4uL P3-A405 15 uL RNA,
- plus 6 uL RT buffer, 3 uL dNTP, 2 uL inhibitor, .2 uL RT
- Master = 36 uL P3-A405 + 54 uL RT buffer + 27 uL dNTP + 18 uL RNAsin + 3 uL RT
Ran oligo cleanup on all 32 new probes. Still need to spec and label these probes. Currently brief labels are on the sides of the tubes and all tubes are in a new cardboard box in my -20C.
STORM
- imaging F03-6 and F02-7 double stains