Wednesday 05/28/14

10:00a – 11:00p

STORM

  • imaging F04F03 samples
  • finish staining / washing B05 + new F03-F04 no secondary, F05-F06 no secondary (see labels on wells)

cell staining

  • Prep 12 new 18mm coverslips for staining
  • Prep 3 new 40 mm coverslips of cells.
  • 1 of 3 40 mm coverslips all cells detached after LN2 step.
  • stain F03-p1 + F04-p3 (check this), no secondaries, on 40 mm coverslips.
  • stain F05-p1 + F06-p3 (check this), no secondaries.
  • stain B06 and B07 (probe prepped yesterday).
  • 40mm coverslips staining 40uL hybe mix + 3-4uL probe, inverted on a regular coverslip and sealed just on 2 edges.

Writing

  • working on Progress Report for Damon Runyon (see googledocs)

project 2

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