10:00 am – 5:45 pm, 8:30 pm – 12:05 am
Lab meeting
- see notes on RC discussion
- see notes on conference update
Other meetings
- project 2 team meeting, 4pm
- chromatin project data update, 5pm
- # send new plots to XZ tonight !
- # send outline of figures to XZ: (1) schema. (2) regions have destinctive features with zoom ins. (3) scaling of area (4) internal domains (5) boundaries (6) model
Chromatin Project
Presentation Prep
- making slides for today’s meeting
Cell staining and imaging
- wash out excess probes D01 – D03 (stained yesterday)
- finish STORM imaging of sample L3B11.
- B11 also lost focus overnight (5am). Fortunately the findsum caught this error and stopped analysis before remaining sections were bleached.
- start imaging sample L3D01
Analysis
- Analyze B09 data through image 21 (53 spots), have 34 images
- Processing B10, B11, F01 and E12 data. E12 mislabeled as E11 in dax files. Running on Tuck and Cajal
- Analyzed F04F02 (black left + En) and F04 + F03 (black left + E(Pc)).
- localization count pretty low.
- 750 channel often quite weak.
Simulations to do:
- simulate random walk polymer, then simulate exponentially distributed counts per spot. See what the effect is on radius of gyration.