Wednesday 06/18/14

9:30 am – 7:30 pm, 10:00 pm – 12:30 am

Tasks

  • Revise figure for XZ

New Library Design

  • Final checks for lib5
  • send Fasta file to Marcelo and request for quote to Alec and Matt

Sequential staining

  • Lib2 F05 + F06.
  • staining S1, 1:500 in hybe dilution buffer, 5:30pm
  • accidently tripped over objective heater, came unplugged. Temp dropped to 28C.
  • replugged (has 2 ports, use front port to go to heater). Heating back to 37C.
  • 6:35pm finish Hybe, washing with 50% Formamide in 2x SSCT.
  • Forgot to add beads. After wash (~6:50pm) remove sample, add beads, replace sample.
  • Focus lock doesn’t work if stage is clipped into the bottom

Simulations

  • tried ultra-stiff polymer (increased K by 1000)
  • started saving template scripts in save folder so we can go back and trace the parameters.
  • Installed dependent 32-bit python 2.6 packages for OpenMM on Monet (as not to interfere with existing 64 bit python 2.7 packages required for storm-analysis and storm-control projects.
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