9:30 am – 7:30 pm, 10:00 pm – 12:30 am
Tasks
- Revise figure for XZ
New Library Design
- Final checks for lib5
- send Fasta file to Marcelo and request for quote to Alec and Matt
Sequential staining
- Lib2 F05 + F06.
- staining S1, 1:500 in hybe dilution buffer, 5:30pm
- accidently tripped over objective heater, came unplugged. Temp dropped to 28C.
- replugged (has 2 ports, use front port to go to heater). Heating back to 37C.
- 6:35pm finish Hybe, washing with 50% Formamide in 2x SSCT.
- Forgot to add beads. After wash (~6:50pm) remove sample, add beads, replace sample.
- Focus lock doesn’t work if stage is clipped into the bottom
Simulations
- tried ultra-stiff polymer (increased K by 1000)
- started saving template scripts in save folder so we can go back and trace the parameters.
- Installed dependent 32-bit python 2.6 packages for OpenMM on Monet (as not to interfere with existing 64 bit python 2.7 packages required for
storm-analysis
andstorm-control
projects.