10:20 am – 11:15 pm
New simulations
- sticky polymer simulations. same as blue simulations but reduce binding energy 10 fold.
index*epsilon * .0001 * (step(r-rm)*(r-rm)^4 * (1- step(r-2*rm))+ (rm)^4*step(r-2*rm)); - now running with 10 polymers, log scaled from 10 to 500 nodes, 3 rpts. Hopefully this will finish before tonight so I can look at it and set up a new batch of simulations.
STORM observations.
- Black domains labeling pretty sparse I think — for an 80 kb region this are pretty dim.
- background is pretty low, probably worth tripling the concentration and seeing if that helps, maybe I’ll try this tonight.
- imaging D06. These regions are WAY larger than D05. Maybe something happened in the genome here. (or the D05 stain failed more spectacularly than I thought, will have to look at the real counts).
PH project
- Analyzing S2 PH data
- sent draft of S2 PH data ANTC data to Ajaz