10:00 am – 5:20 pm, 10:00 pm – 11:50 pm
Goals
- prep new cells for multicolor hybe
- continue analysis of E7 data
Lab meeting
- Ruobo presentation – see protected notes
- Jiang journal club — axon mylination in response to neural signaling
Project 2 analysis
- see analysis of E7
- see summary E7
Cell prep
- prepping cells fixed yesterday for hybridization
- in RNase treatment in warmroom (Hao is using new hybe oven and probably best to keep that RNase free)
- now in 50% formamide at 4C ready to hybe.
new stains
- 2 large coverslips of BXC P1 + P3 for double labeling control against movement
- 1 large coverslip of L3Eo6toE09 for double labeling control against movement
STORM
- imaging L3E09 PH in S2 cells
- 647 switching looks much better than when imaged with BX-C. Maybe should repeat BX-C
- 750 staining looks great but deactivates quickly
- might try more imaging of this slide with 750 STORM. should probably do in MEA instead of BME
- use mosaic tile to get conventional images if STORM is already inactivated (just match stage location?)
- need more COT