Tuesday 07/29/14

9:00 am – 5:30 pm, 9:00 pm – 12:30 am

Goals

  • discussion with Chong about project
  • washout probes from new hybes
  • break down STORM run, transfer and fit data
  • spot brightness analysis
  • flip fly stocks (?) — not managed

Chromatin project

New library design

  • designing new probes for En and B09 (yellow ~100 kb) that alternate probes with different primers
  • will synthesize these with P2 and P3 primaries.

Sequential staining control

  • imaging BX-C-p3-A647

New Stains

  • E08-p1-A647 + E09-p3-A750
  • F06-p1-A647 + F07-p3-A750
  • F07-p1-A647 + F06-p3-A750

Project 2

  • see summary
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