10:15 am – 8:00 pm
- out of RNasin, out of Maxima, not able to proceed with probe making.
- really need to have back ups of these.
- started to write SteveMosaicViewer GUI
- drag and drop of a mosaic file will create run
ConvertStv2Matif needed and create a
stvfileglobal that can be loaded
- rest of render still needs work
- fixing issues with Mosaic tiling x,y definitions, transposes etc.
- odd issue with tiling: overlapping info:
- imaging E06 E07 two color
- same buffer as yesterday: 1.5 uL BME, 2.5 uL MEA, 5 uL COT, 10 uL GLOX, 500 uL im buffer with glucose.
- spots look pretty good.
- mosaic scanned using 647 channel.
- now recording channel in filename
- (.5 uL of secondary and 2 uL of primary each)
- L2 F01-P3-A750 + F02-P2-A647
- L2 F03-P3-A750 + F02-P2-A647
- L2 F05 + F06
- L3 E08 + E09