New Findings from Previous Goals:
1. Snail minus shadow rescues: Some embryos don’t gastrulate at all w/o shadow enhancer. Preliminary results show some still do gastrulate. Getting the numbers up next.
2. Stat sig. testing hb/kr/kni: see latest draft. The p-values will hang on every comparative statement like ill-tempered sharp-toothed mice.
3. Heterogeneous accumulation snail +/- enhancers: Developing more robust algorithm for separating dots in 3-D. Each dot is localized in several consecutive z-sections and reconstructed into a 3D PSF. (Processing ~0.3 million dots per-embryo requires a whole new method than what I had working for a couple thousand dots).
Priorities for Next Month:
1. Finish draft of gap shadow paper (with new organization, new stats, new detailed methods).
2. Get 3D dot segmentation code working robustly.
3. Complete thorough comparison of total snail transcript +/- primary (to present along with ‘single-molecule’ imaging method at Fly Meeting?).