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Tag Archives: images
Compare 2D and 3D spot finding results
2D in green, 3D in red (H3-647). Z images are collapsed down into single plane. It looks like brightness differences are substantial, better contrast total photons from 2D imaging (might have expected reverse, 3D apparently doesn’t compensate for photon … Continue reading
Posted in Chromatin, Microscopy
Tagged image analysis, images, STORM
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Protected: Super-resolution imaging of PcG bodies
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Posted in Microscopy
Tagged confocal, images, STORM
Comments Off on Protected: Super-resolution imaging of PcG bodies
Protected: 3D optics in thicker sections
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PcG bodies
PcG bodies of Pho and Psc.
confocal test summary
confocal of:
Protected: PcG in mitosis summary
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Protected: H3K4me3 by confocal and STORM
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whole-mount tests 02/09-10/12
slide H1: 4 color anti-nuclear lamin test slide H2: Directly labeled antibodies: Dm01-488(green) H3K27me3-Cy3B (red), H3-Cy5 (blue), H3K27ac-750 (not shown) slide H4: H3K27me3-Cy3B (red) and H3K4me3-Cy5 (blue) along with 405 background (green). slide H4: earlier embryo: H3K27me3 ‘clusters?’ (red) … Continue reading