Tag Archives: drift

Thursday 02/13/14

Posted on February 13, 2014 by admin

9:30a – 12:40a Goals Today Start Bowtie analysis of Deep-Seq data Apply new drift correction approach to STORM data, compare spot quality STORM imaging of PH clusters + DNA FISH if anti-PH fails, try to restain with anti-Flag nuclear signal … Continue reading →

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Wednesday 02/12/14

Posted on February 12, 2014 by admin

9:50a – 11:59p Ph Project Cell staining hot wash post-hybe PBS wash Block 1 hour in 2% BSA primary stain rb a-Ph for 2 hrs RT, 6 hrs 4C rinse, wash in PBS, reblock, secondary stains O/N at 4C (m … Continue reading →

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Saturday 01/04/13

Posted on January 4, 2014 by admin

10:00a – 11:55p (sailing canceled do to malfunction of committee boats) coding matlab-storm RunDotFinder “skip existing” option on DaoSTORM I believe is interpreted as resume and in insightM as actually skip. This should be double checked and made clearer. Maybe … Continue reading →

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Wednesday, 01/01/2014

Posted on January 1, 2014 by admin

1:30p – 9:10p, remotely, 12:30a – 1:15a Goals more Ph imaging Wt New Year’s letter website updates Troubleshooting drift correction curves. start working on dual-color ChromatinCropper STORM for Ph project The buffer has all gone, because I ran the thing … Continue reading →

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