Tag Archives: image analysis

Sunday 07/29/12

Posted on July 29, 2012 by admin

9:30 A- 10:00A, 11:30A-6:30P Rerunning multi-label counting controls and probe competition experiments. control 1 is tiled with multiple red and green probes.  control 2 competes two short, contiguous probes for the same sequence.  Since these rarely manage to give overlapping … Continue reading →

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chromatic warp issues

Posted on June 6, 2012 by admin

warped image blurs out certain channels.  here 561 gets stretched along x. In the K27/K4 dataset 750 is dramatically stretched along x-y in a correlated fashion. Distortions get worse using higher order polynomial (e.g. 4th order).   Distortions are no … Continue reading →

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Saturday 04/21/12

Posted on April 21, 2012 by admin

10:45 A – 7:45 P, 10:30P – 11:30 P Working on lab meeting presentation slide organization new analysis of Psc, H3K27, H3 localization differences HP1a doesn’t haven’t correct conventional level nuclear localization Samples to cut and image 4H  — K27me3-750, … Continue reading →

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Friday 03/20/12

Posted on April 20, 2012 by admin

11:55 A – 8:00P, 9:30 – 11:30P (volleyball 8:50A-11:50A) need missing reference letter and electronic submission for ASC app! running STORM analysis on 04/05/12 En-tou-Epc-H3 data. Rerunning chromatic alignment on 04/14/12 en-Epc conventional data working on slides for group-meeting presentation

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Thursday 04/19/12

Posted on April 19, 2012 by admin

9:50 A – 8:00P, 9:30 – 10:30P Working on group meeting presentation background slides figure out and implement gaussian dot fitting in matlab use precise fitting to locate molecules in conventional images of En-Epc-H3 stained embryos, superimpose chromatically corrected images. … Continue reading →

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Wednesday 04/18/12

Posted on April 18, 2012 by admin

9:45 A -8:00P, 9:30P-11:00P Running alignment on all E(Pc)-en-H3 data Used H3 4-color experiment beads.  should use correct slide beads. Running bead analysis for all E(Pc)-en-H3 data. Create new fxns for bead runs.

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Compare 2D and 3D spot finding results

Posted on March 2, 2012 by admin

2D in green, 3D in red (H3-647).   Z images are collapsed down into single plane.  It looks like brightness differences are substantial, better contrast total photons from 2D imaging (might have expected reverse, 3D apparently doesn’t compensate for photon … Continue reading →

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Monday 02/20/12

Posted on February 20, 2012 by admin

9:45 A — 10:00 P Transfer embryos into 30% resin Group meeting / BPS practice talks Transfer embryos into 50% resin New collection cages: 4x snail, yw, and MP09s. embryos into 70% resin.  3 hrs, then 100% resin. images uploaded … Continue reading →

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