Tag Archives: polytene

Saturday 12/08/12

Posted on December 8, 2012 by admin

11:00 A – 7:30P, 8:45P – 10:00P Cell staining label with m-HP1a -405,750 and rb-H3K27me3 -488 (top) or rb-H3K27ac -488 (bottom row) Polytene cell staining label with m-HP1a-Cy3b and rb H3K4me3 -488 or rb H3K27me3-488. check staining on confocal some … Continue reading →

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Wednesday 12/05/12

Posted on December 5, 2012 by admin

9:30 A  – 7:40P, 9:00P – 12:00A Confocal dot distances Finish O/N confocal run of Ubx-AbdA data Probe making sent order to LC Sciences for chromatin types On why previous probe might have failed:  over drying? — DNA and RNA … Continue reading →

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Wednesday 11/27/12

Posted on November 28, 2012 by admin

10:15 A — 11:15 PM Polytene cell staining  hot washes in 2x SSCT (45 C in coplin jar in dry oven) check staining — cent probe looks good (rather small foci though) Antibody staining H3K9 did not work.  Should have … Continue reading →

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