Author Archives: admin

Saturday 06/07/13

11:00 – 5:30P Project 2 see notes OligoPaint library code Stupid transcriptome libraries necessarily have multiple copies of each gene for all the splice form variants. OligoArray returns these all as hits, even when their genome coordinates overlap. finished BLAST … Continue reading

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Protected: project 2 06/08/13

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Friday 06/07/13

10:30 A – 11:30 PM Project 2 see notes Probe condition optimization correctly identified sample types STORM laser powers may not be optimal, see observations under STORM HPLC HPLC training with Bryan STORM Finish O/N STORM2 run of BX-C BB3 … Continue reading

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Protected: project 2, 06/07/13

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Thursday 05/06/13

10:30A – 7:30P, 9:00P-12:45A project 2: Team meeting, 2PM notes and planning Clarification meeting with XZ more cell staining Presentations putting rough slide drafts together for Wu lab presentation taking slides from non sensitive data for SDB presentation STORM STORM … Continue reading

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Protected: project 2: experiment planning

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Protected: project 2

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Wednesday 06/05/13

10:00 A – 12:30 A Project 2 see private notes. Computer Installed new RAID50+ usb 3.0 hard-drive array OligoPaint STORM testing sample BB3. substantially more responsive to 405. More switching (65K frames recorded instead of 45K with exhaustive acquisition).

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Protected: project 2 team meeting

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Tuesday 06/04/13

10:00 A – 12:30 A cell staining heating wash buffers to 47C imaged 20 bp single probe cells (5 cells). imaged 40 bp array (with 0 gain, oops). (6 cells) imaged 40 bp array for restain (7 cells) Doory imaging … Continue reading

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