Author Archives: admin

Thursday 03/14/13

Posted on March 14, 2013 by admin

10:00 A – 7:40P, 8:30P-10:30P Genomics processing real vanSteensel data now Reading in data and converting to int8 data matrix for data compression.cellfun(@(x) int8(round(20*str2double(x))), data) seems to be running slower than looping the over the columns… Actually need single to … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Thursday 03/14/13

Ethan Garner Seminar 03/14/13

Posted on March 14, 2013 by admin

lab running for ~6 months now. Introduction bacterial cell organization (in rod like cells): ends, middle, equally spaced ParM finds the ends by pushing — polymerizing until it starts bending. How bacteria grow cell wall holds shape. Digest this, get … Continue reading →

Posted in Seminars | Comments Off on Ethan Garner Seminar 03/14/13

Wednesday 03/13/13

Posted on March 13, 2013 by admin

10:00 A – 9:30P, 11:00P – 12:55 A STORM Finish O/N run on STORM2 STORM Analysis Launch Analysis of all fab7 data collected on STORM2 — now running on Cajal in small batches. Finish configuring local repo on TUCK to … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Wednesday 03/13/13

multicolor by sequential staining

Posted on March 13, 2013 by admin

Approach Objective and Adventages Avoid some of the incompatiability issues of protein-DNA staining: Protein stains in previously untreated tissue should always work DNA stains should still work after protein imaging — it’s just the formamide treatment tends to kill the … Continue reading →

Posted in Research Planning | Comments Off on multicolor by sequential staining

Tuesday 03/12/13

Posted on March 12, 2013 by admin

10:00A – 11:15P STORM Finish O/N STORM run on STORM2 Finish O/N STORM run on STORM3 Installing mount and sliders on STORM4 to allow for 3D imaging Lessons: Final appeture (removed for alignment) front-back position chosen to minimize the size … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Tuesday 03/12/13

Monday 03/11/13

Posted on March 11, 2013 by admin

10:00 A Group meeting (Eileen) see notes Journal club: High salt recruits aversive taste pathways STORM Hal crashed on STORM3 at 10:11A (still 34 locations acquired). sent STORM3 error report to Hazen (see email record) STORM2 run seemed to finish … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Monday 03/11/13

Journal club: High salt recruits aversive taste pathways

Posted on March 11, 2013 by admin

Nature, recent Presented by Guisheng 5 classes of taste receptors for umami, sweet, bitter, sodium and sour and carbonation each taste bud contains different cell types containing different receptors tongue map is wrong — each tastebud has all cell types. … Continue reading →

Posted in Journal Club | Tagged | Comments Off on Journal club: High salt recruits aversive taste pathways

Protected: lab meeting 03/11/13

Posted on March 11, 2013 by admin

There is no excerpt because this is a protected post.

Posted in Lab Meeting | Comments Off on Protected: lab meeting 03/11/13

Protected: Alternative library and probe designs

Posted on March 10, 2013 by admin

There is no excerpt because this is a protected post.

Posted in Chromatin, probe and plasmid building | Tagged , | Comments Off on Protected: Alternative library and probe designs

Sunday 03/10/13

Posted on March 10, 2013 by admin

9:30 A – 12:15 A Meeting with Ajaz oops it’s daylight savings day! Ajaz will induce and fix cells, bring over fixed cells on coverglass label with m-anti-Flag + A647-A405-dk a-m secondary and STORM. Compare clusters. STORM Finished O/N imaging … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Sunday 03/10/13