Author Archives: admin

Emulsion PCR

Posted on February 28, 2013 by admin

For initial amplification from Oligo-Library. Setup Components Glass vials kept at -20C. magnetic stir plates at 4C/in cold room (capable of 1000 rpm) emulsion oil mix (can make in 50mL volume and store at 4C): 80 uL ABIL EM90 (request … Continue reading →

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Filtering interval data

Posted on February 27, 2013 by admin

Allowing small gaps. Some very brief changes in chromatin type may not be real: Need to allow gaps – small misclasifications rrrrrrrrrrr kk rrrrrrrrrrrrrrrrr bbb rrrrrrrrrrr should still be scored as a long red region. The kk/bbb might be background, … Continue reading →

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Wednesday 02/27/13

Posted on February 27, 2013 by admin

9:00 A – 10:00 P Embryo collection Flipped Esc cage — good looking overnight collection Probe design schematic of probes design and send to Brian new probe sequences for Custom Array order. working on new probe sets for colors project … Continue reading →

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Protected: probe design

Posted on February 27, 2013 by admin

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Tuesday 03/26/13

Posted on February 26, 2013 by admin

10:00 A – 6:30P, 7:50P – 10:20P Embryo work Flip collection cages ~10:00 A No hatching from Mat apha tub x Rad21, Scm, Pc(3), Psc(1) shRNAs. all these embryos appear unfertilized. Frequently moderate deformaties to egg shape, substantial variation in … Continue reading →

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Monday 03/25/13

Posted on February 25, 2013 by admin

9:45 A – 7:30P, 8:45P-12:30A Embryo collection Flip ESC embryos Flip all other embryos (w/o yeast, just as a holding pattern). check vitality — Psc1 has a 5 of 100s hatched, others have 0 hatched yet. Will give them another … Continue reading →

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Protected: lab meeting 02/25/13

Posted on February 25, 2013 by admin

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Sunday 03/24/13

Posted on February 24, 2013 by admin

11:30A – 5:00P, 6:30P – 8:15P Coding Overhauling all file loading for STORMrenderBeta Single Bin files Loaded through open menu (or toolbar) Multiple Bin files, manual select Prompts user to find a chromatic warp file Allows warp dimension to be … Continue reading →

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Saturday 03/23/13

Posted on February 23, 2013 by admin

10:30 A – 6:20P, 9:30P Embryo collection Flip all fly cages dispose of previous fly plates with new plate recycling and freezing system. Fix embryos 9:30 PM (~3-14 hrs, mostly at raised at 29C). STORM working out parameters for TCEP … Continue reading →

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STORM Buffers

Posted on February 23, 2013 by admin

TCEP Buffer 0.2M Tris pH 9  (200 uL Tris 1M pH 9.0) 1 mM Methyl viologen (10 uL of 0.1 M stock.  Currently make fresh from powder. Toxic) (25.7 mg / mL) 1 mM Ascorbic Acid (10 uL of 0.1 … Continue reading →

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