Author Archives: admin

Monday 06/29/15

Posted on June 29, 2015 by admin

9:45 am – 5:15 pm Testing RNAi knockdown RNA isolation (assuming you cultured in 6 well plates – scale accordingly) * Wash cells in cold PBS (with Kc, might have to resuspend, spin down, wash in PBS, then spin down … Continue reading →

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Protected: Sunday 06/28/15: ASBMB day 4

Posted on June 28, 2015 by admin

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Protected: Saturday 06/27/15: ASBMB day 3

Posted on June 27, 2015 by admin

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Protected: Friday 06/26/15: ASBMB day 2

Posted on June 26, 2015 by admin

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Protected: Thursday 06/25/15: ASBMB day 1

Posted on June 25, 2015 by admin

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Wednesday 06/24/15

Posted on June 24, 2015 by admin

9:50 am – 8:00 pm presentation prep tuning talk and slides Coding rewriting Chromatin Cropper New Organization 100% of the action of the GUI should be executable as command line functions (currently the main processing “steps” are still steps of … Continue reading →

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Tuesday 06/23/15

Posted on June 23, 2015 by admin

9:40 am – 11:00 pm Heat shock experiments continue cell prep LN2 treatment, 5 min 1M HCl bath RNase A digestion (30 min at 37C) SSCT wash de-ionizing fresh formamide prepared new formamide buffers prehybe and store at 4C Bogdan … Continue reading →

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Monday, 06/22/15

Posted on June 22, 2015 by admin

10:00 am – 5:15 pm 10:45 pm – 11:30 pm Tasks discussed with Dell again about memory (they make this unnecessarily complicated). Experimental goals for today check progress RNAi knockdowns purify more RNA for RNAi set up new RNAi knockdown … Continue reading →

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Sunday 06/21/15

Posted on June 21, 2015 by admin

9:50 pm – 11:45 pm RNAi RNA prep set up T7 reactions 10 x 9 = 90 uL NTP buffer mix 2 x 9 = 18 uL T7 pol mix 2 x 9 = 18 uL RNasin 6 x 9 … Continue reading →

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Friday 06/19/15

Posted on June 19, 2015 by admin

9:30 am – 5:00 pm Meetings lab meeting (see notes) journal club (see notes) RNAi prep dsRNA template gel (should poor new gel, lanes running funny in old reheated gel. sample order: 1-8 = (Kc-DNA): Su(z)12, SCE, Ph-p, Ph-D, (YW-DNA): … Continue reading →

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