Author Archives: admin

Monday 09/15/16

Posted on February 15, 2016 by admin

10:07 am – Tasks Discussion about super-resolution of individual membrane proteins (EL lab). Reply to request for data from JM Victor. Project updates with BB. Need to contact LM team about explaining model. Discussion on quantifying nuclear structures with new … Continue reading →

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coding

Posted on February 7, 2016 by admin

Polishing MERFISH code for public release. Plan take 140 gene library, create short fasta of just complete genes, run it through Launch OligoArray add parse OligoArray (as a function) into this script add assemble secondaries and target regions into as … Continue reading →

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Friday 02/05/16

Posted on February 5, 2016 by admin

9:30 am – communication discussion with Paul on MERFISH Microscope building updating parts list discussion with Hsuan on plans see shared dropbox folder and shared excel files (need to link in) Interview Prep prep for Salk and MCB interviews

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Wednesday 02/03/16

Posted on February 4, 2016 by admin

10:00 am – 5:00 pm Probe synthesis start synthesizing 2kb probes (with SW secondaries and Fast secondaries) running T7 reaction O/N. To do test SDS treatment of embryos test 2 kb probes (scope time on Friday evening). Results from yesterday … Continue reading →

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Thursday 02/04/16

Posted on February 4, 2016 by admin

12:00 pm – 6:30 pm (10:00 am – 12:00 pm working remotely on library). Library prep updated 15 kb en library to use F primer L6F5 with L6R2 instead of F1, which I already used in lib7 ordered en 15 … Continue reading →

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Tuesday 02/02/16

Posted on February 2, 2016 by admin

8:45 am – 10:40 pm schedule Rinse out probes, set up primary hybe for bit 1 for embryo staining experiment tracing BX-C Wu lab group meeting meeting with Scolnick at Broad Garner seminar on academic advice (see protected notes) continuing … Continue reading →

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Protected: Group meeting 2/2/16

Posted on February 2, 2016 by admin

There is no excerpt because this is a protected post.

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Protected: Dan Norcea: running a lab

Posted on February 2, 2016 by admin

There is no excerpt because this is a protected post.

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Protected: Ethan: Packing your parachute: Career Advice

Posted on February 2, 2016 by admin

There is no excerpt because this is a protected post.

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Monday 02/01/16

Posted on February 1, 2016 by admin

9:05 am – 7:30 pm, 8:15 pm – 11:40 pm Imaging testing control L4E24 embryos. stains look great. compare to L7 embryos — no staining. ran series removing S2 with toe-hold 5 bp. Looks excellent. Troubleshooting Gel tests try to … Continue reading →

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