8:45 am – 10:40 pm
schedule
- Rinse out probes, set up primary hybe for bit 1 for embryo staining experiment tracing BX-C
- Wu lab group meeting
- meeting with Scolnick at Broad
- Garner seminar on academic advice (see protected notes)
- continuing hybridization experiment
- Norcea seminar on running a lab (see protected notes)
- continuing hybridization experiment
- work on equipment cycle request for new scope
Embryo staining
- RNA template non-degradation was indeed the problem, beautiful staining in bit 1.
- bit 1 removes nicely in 20 min.
- adding bit 2, background still very high after 5 min wash, running longer wash, more fluid changes.
- imaging 4% 647 laser at 150 mW.