12:00 pm – 6:30 pm
(10:00 am – 12:00 pm working remotely on library).
Library prep
- updated 15 kb en library to use F primer L6F5 with L6R2 instead of F1, which I already used in lib7
- ordered en 15 kb library along with probes for BB at 12K scale from CustomArray
- also sent MC our recent paper on chromatin imaging
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Library name: L8_SI6.fasta
- library organization:
% 3′ P4-Alexa405, A647-Steven,
% 5′ FwdIndex, cy3-commonRT, TruncStevenSecondary, targetRegion, RevIndex
% 20-bp, 20 bp, 20 bp, 42 bp, 20 bp, total: 122
Embryo staining
- label embryos
New libraries in consideration
- small MERFISH library to drosophila embryonic genes
- eve locus at 2 kb resolution
- cut locus at 5 kb resolution
Probe synthesis
- continued RT for 2kb resolution probe library against BX-C region
- updating protocol
Other
- booked flight back from SFO to BOS on Sat Mar 5th.
Received
- L7S (L7-secondary) arrived today
- S1-L7common fusion arrived today
Microscope building
- Hsuan offers to help. great!