Author Archives: admin

Sunday 10/13/13

Posted on October 13, 2013 by admin

11:00a – 7:00p Ph Modeling chances = 0 if PhM >= PhWt This version finally gives the appropriate trend of clustering as a function of PhM concentration the tails are still too long, probably needs to be fixed by finite … Continue reading →

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Saturday 10/12/13

Posted on October 12, 2013 by admin

3:00p – 7:50p STORM Stop O/N STORM run of 475 kb black region F10 spot flickering not looks dimmer make new Glox test new hybes: G3/G4 failed. F12 failed. F9/G3 failed. Possible reasons for failure: suspect extended hot wash in … Continue reading →

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Friday 10/11/13

Posted on October 11, 2013 by admin

10:00a – 10:30p Goals send Ph modeling results to Ajaz work on presentation send reminder about racing Saturday STORM analysis Copying F11 data to ProBox2. Delete data from Storm2 Running splitdax on no405Primary, E07fail, F09, and F01v2 (latter 2 including … Continue reading →

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Protected: Ph Meeting Notes

Posted on October 11, 2013 by admin

There is no excerpt because this is a protected post.

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Thursday 10/10/13

Posted on October 10, 2013 by admin

10:00a – 8:30p, 10:00p – 12:00a Goals finish abstract for Alvaro work on slides for Stowers STORM with Bogdan finish F1, start coverslip 4 STORM data processing with Bogdan Oligo Prep G3-G5 P1A405, P2Cy3, P4Cy3 samples Hybridizations for next small … Continue reading →

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Abstract for Seminar at Rowland Inst.

Posted on October 10, 2013 by admin

Title: Quantification and Analysis of Intrinsic Variation in Gene Expression during Embryonic Development Alistair Boettiger Harvard University Abstract: The molecular scale interactions responsible for turning genes on and off have an inescapable degree of randomness — a consequence of their … Continue reading →

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Wednesday 10/09/13

Posted on October 10, 2013 by admin

9:00a – 12:35a Goals work on Presentation for Stowers STORM Ran out of disk space, crashed O/N Run lost focus anyway immediately after position 0. Probably not enough oil. Possibly positions were chosen too far apart. Need to explain these … Continue reading →

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Revised Fast OligoPaint Synthesis

Posted on October 9, 2013 by admin

Jeffrey Moffitt, Alistair Boettiger, Xiaowei Zhuang jeffmoffitt@gmail.com alistairboettiger@gmail.com Version 2.0: October 2013 Purpose and Overview This protocol describes the construction of OligoPaints FISH probes from a complex oligo library via reverse transcription of an RNA intermediate. By exploiting the high … Continue reading →

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Tuesday 10/08/13

Posted on October 8, 2013 by admin

9:40a – 10:00p Goals Stop O/N STORM run send code to Jeff (11a, dentist) Talk title for Alvaro STORM Stop O/N STORM run IVT reactions out of High Yield T7 buffer Still have 2 vials of T7 High Yield Pol … Continue reading →

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Monday 10/07/13

Posted on October 7, 2013 by admin

9:45a – 8:00p, 1:00a – 2:00a General Group meeting cancelled XZ meeting cancelled Ph modeling Discuss analysis methods with Jeff Moment comparisons. nmin / cv^2 way different than 1, definetely not exponentional (also obvious by visual inspection, median 4-7, mean … Continue reading →

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