Author Archives: admin

Saturday 09/14/13

Posted on September 14, 2013 by admin

Zenklusen Intro use nascent mRNA to measure initiation frequency live imaging short memory of how many transcripts initiated MS2 RNA labeling — look at intensity fluctuations initiations are uncorrelated. variable elongation rates coordinated with cell cycle (faster in G2/M) MS2 … Continue reading →

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Thursday 09/12/13

Posted on September 12, 2013 by admin

STORM analysis set up splitdax analysis for last of unsplit G4 data (somehow a lot of 0 bit movies got written). set up DaoSTORM analysis for G4 data Travel to Banff for BIRS conference Writing reading latest paper for review … Continue reading →

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Wednesday 09/11/13

Posted on September 11, 2013 by admin

9:30a – 11:30p Review submit review submit copyright agreement BIRS draft email of final reminders contact Vistas Dining hall about meal costs and reserving tables Print driving directions to and from Airport/Banff, Lakes, and hotel. Print hotel receipt Probe making … Continue reading →

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Tuesday 09/10/13

Posted on September 10, 2013 by admin

8:50a – 4:40p STORM Finish O/N STORM of G4 start splitdax Probe making RNA cleanup using Beckman RNAClean XP beads. Samples F1-F5, F7-F9. Tube format F1 yield ~1,500 ng/uL in 25 uL (37.5 ug of RNA). F2 yield ~700 ng/uL … Continue reading →

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Monday 09/09/13

Posted on September 9, 2013 by admin

9:00a – 7:30p, 8:00p-11:30p Goals Re-read and submit review! slides for meeting with XZ. Meetings lab meeting journal club project 2 meeting (see posts) File management delete raw dax for movies separated by splitdax copy files Review MiKTeX updater broke … Continue reading →

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journal club

Posted on September 9, 2013 by admin

(Sebastian presenting) Paper Lang … Chin Nature chemistry 2012. currently method of choice in the field. background site specific labeling of single cystine. Hard to remove extra Cys Fluorescent protein fusions (large, may have major functional impacts). self-labeling proteins: SNAPtag, … Continue reading →

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Protected: lab meeting 09/09/13

Posted on September 9, 2013 by admin

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Sunday 09/08/13

Posted on September 8, 2013 by admin

Goals Run gel to test RT reactions from plate-based T7 (complete) second proof read of review article, prepare to submit (not complete) STORM imaging (complete) Review started rereading and editing replies to reviewers. Results E6, E7, E10, F6 with P1 … Continue reading →

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Friday 09/06/13

Posted on September 6, 2013 by admin

8:50a – 5:10p, 8:30p – 10:40p Other stuff write to landlady about move in return keys for 2nd floor Coding fixed bug in STORMfinderBeta.m For Hao for updates: Fork fast-forward https://github.com/blog/266-fast-forward-your-fork Data Analysis copying files to ProRAID while running splitQVdax … Continue reading →

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Protected: project 2: 09/05/13

Posted on September 5, 2013 by admin

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