Author Archives: admin

Tuesday 07/16/13

Posted on July 16, 2013 by admin

10:00 A – 5:30 P STORM Finish O/N STORM of S3 repeated (not sure why contrast was low on first round). computer maintenance: update software, reboot system, relaunch all aborted STORM analysis scripts ProRAID required switching off during second reboot … Continue reading →

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Monday 07/15/13

Posted on July 15, 2013 by admin

9:30 A – 7:45 P, 8:30 P – 10:30 P STORM + analysis finish O/N STORM run of no primary control. may have lost focus (might have been just the final return to start) Set up new O/N STORM on … Continue reading →

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Protected: lab meeting 07/15/13: PD interview

Posted on July 15, 2013 by admin

There is no excerpt because this is a protected post.

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Sunday 07/14/13

Posted on July 14, 2013 by admin

9:40 A -3:00 P, 9:00 P – 10:30 P STORM Finish O/N STORM of BXC-S4 copy data # start analysis start setup for STORM of BXC-S5 better switching than S1-4, higher background in conv channel than S1 and 3 Progress … Continue reading →

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Saturday 07/13/13

Posted on July 13, 2013 by admin

10:00 A – 3:00 P STORM finish STORM imaging of BXC-S3. start STORM imaging of BXC-S4. Observations: first 10K frames of switching look pretty bad contrast high background ~10K-65K frames have much better contrast and reasonably good switching. Not highly … Continue reading →

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Friday 07/12/13

Posted on July 12, 2013 by admin

9:30 A – 4:00 P Discussion 9:30 A – 11:30 A, meeting with Alvaro Sanchez discuss differences in noise regulation from bacteria through mammalian cells and reasons for these different regimes. discussed repressor vs. activator noise, better to have the … Continue reading →

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Chromatin Experiments To Do

Posted on July 11, 2013 by admin

in situs BX-C Expression and DNA locus multiplex whole-mount embryo in situs for AbdB, AbdA, and Ubx with dig probes anti-dig-405,647 ultra cryo section embryos. label with WGA-488 on coverlgass, then STORM the RNA labels. DNA-FISH BX-C or individual AbdB, … Continue reading →

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Thursday 07/11/13

Posted on July 11, 2013 by admin

10:00 A – 7:00P, 8:30P-10:00P STORM Finish O/N run of PhM-flag-488, Ph-647 take bead images transfer data to ProRAID Set up analysis to run on Cajal Embryo in situs wash out probes check staining: All embryos washed off! 1 tiny … Continue reading →

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Wednesday 07/10/13

Posted on July 10, 2013 by admin

10:00 A – 7:40 P, 8:30 – 10:00 P General laboratory safety training refresher check HoxD server jobs: still heavily backed up check STORM analysis / drift correction: Y complete. Others still running. update notebook entries from yesterday. Review working … Continue reading →

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Protected: project 2 07/10/13

Posted on July 10, 2013 by admin

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