10:00 A – 3:00 P
STORM
- finish STORM imaging of BXC-S3.
- start STORM imaging of BXC-S4. Observations:
- first 10K frames of switching look pretty bad contrast high background
- ~10K-65K frames have much better contrast and reasonably good switching. Not highly 405 responsive but appreciable effect.
Literature
- Reading Pirrotta’s latest PLoS genetics on Tx and Pc factory targeting (finally).
- ‘targeting’ based on colocalization of transgenic fab7 alleles when active or repressed.
- dependent on CTCF.
- CTCF null mutants (no CTCF in polytene squash) still make flies — 50% still eclose, but die within 24 hours (pharate stage, never sexually mature). (see Mohan et al 2007 CTCF paper, very thorough).
- (CTCF still contributed maternally)
Review
- writing Markov section introduction