Category Archives: Research Planning

Levine Progress Reports, planned experiments, research summary

Saturday 03/23/13

Posted on February 23, 2013 by admin

10:30 A – 6:20P, 9:30P Embryo collection Flip all fly cages dispose of previous fly plates with new plate recycling and freezing system. Fix embryos 9:30 PM (~3-14 hrs, mostly at raised at 29C). STORM working out parameters for TCEP … Continue reading →

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Protected: Meeting Notes

Posted on February 5, 2013 by admin

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Oligo Paints Goals 01/30/12

Posted on January 30, 2013 by admin

In situs single color BX-C-cy5 in S2R+ cells (done) BX-C-cy5 in S3 cells (image: 01/30/13) Fab7-cy5 + 405 activator in S2R+ (image: 01/31/13?) Ubx-cy5 in S2R+ (?) Abd-B in S2R+ cells (not yet stained) Abd-B in S3 cells (not yet … Continue reading →

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Research goals

Posted on January 16, 2013 by admin

Oligo Paints Week of 01/15/13: synthesis en-Cy5 probe (by my hands in Zhuang lab and by Brian’s trainee at HMS) and of BX-C locus (by Brian). Monday-Tuesday (01/21-22/13), test in situs of en and BX-locus Tuesday: section Drosophila yw embryos … Continue reading →

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Research Planning 12/14/12

Posted on December 15, 2012 by admin

Use antibody accessability as a proxy for PolII accessability. Compare levels of staining of H3K27me3 in intact vs. mechanically spread nuclei.  (First we need to see if H3K27me3 even remains associated in spread nuclei.  Try spreading nuclei using the ‘shearing’ … Continue reading →

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Protected: Notes from meeting with Welcome Bender

Posted on November 9, 2012 by admin

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Protected: Francis Lab Meeting feedback

Posted on November 7, 2012 by admin

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Protected: snail project further experiments

Posted on November 7, 2012 by admin

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Protected: summary results so far on K27me3 dynamics

Posted on October 18, 2012 by admin

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Protected: Experiments to try later

Posted on October 18, 2012 by admin

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