Oligo Paints Goals 01/30/12

In situs

single color

• BX-C-cy5 in S2R+ cells (done)
• BX-C-cy5 in S3 cells (image: 01/30/13)
• Fab7-cy5 + 405 activator in S2R+ (image: 01/31/13?)
• Ubx-cy5 in S2R+ (?)
• Abd-B in S2R+ cells (not yet stained)
• Abd-B in S3 cells (not yet stained)
• En in S2R+ cells (not yet stained)
• BX-C in embryos (stain on: 01/30/13)
• En in embryos

multi-color

• Abd-B-cy5, BX-C-488 (not yet stained)
• BX-C flanking region -cy5, BX-C-488 (not yet stained)
• color-flip of both of these

Multiplex Protein

• BX-C labeled sections, m-dm01-Cy3B, rb-H3K27-abcam-750.
• Fix, NaBrH[4] treat, then m-dm01-Cy3B, rb-H3K27-abcam-750. then BX-C-cy5 label
• color flip BX-C-488, rb-H3K27me3-647
• embryos, m-Ubx / m-AbdA / m-AbdB treated,
• embryo sections, En-Protein + dm01 direct label. 3 min post fix in PFA/GA (4% / 0.1%) Then prep for EN-locus in situ.

Probes to make:

• BXC unlabeled primary (Brian will make)
• En-locus unlabeled primary
• 2R YELLOW DNA 110 kb locus, emPCR

Request from Brian

• BX-C emPCR product – to make secondary label-ready probes?
• BX-C labeled