10:00 A – 7:20P, 8:45P – 11:45P
General
- As of Today, all IDT oligo orders are to go through Matt, not through HHMI card on file.
Cell labeling
- heating up 2X SSCT wash to 60C in large hybe oven
- check staining (cells detached onto the slide).
- continuing on with antibody labeling: coverglass mounted on stands in humidity chamber.
- Mounted En slide for STORM. Don’t find dots in cy5 channel? Test on confocal tomorrow? recheck original slide?
labeling antibodies
- Started new page for new box of antibodies
Fly Work
- Collect virgins
- crossed da[10]/Inv-Gla x esc[6]/CyO virgins
- (hoping to make an esc[6]/Inv-Gla stock, which save a cross in making esc M/Z null embryos. Not sure if Inv-Gla is a sufficient balancer for esc[6], will have to research this one).
- PM virgin collection
Coding
- Switched Lab-Notebook entries into Markdown. Quality.
- matlab-storm repo active and synched
- deleted drop-box matlab-storm folder (now managed through Git)
- Reconfiguring filepaths on Tuck to use Git to keep local repo copy up to date.
- Test run of L1_Homotopy following Readme from Matlab fails (cvx_quiet not found).
- Test run of L1_Homotopy from command line
- Testing DaoSTORM cd DaoSTORM_folder c:\Python27\python.exe mufit_analysis.py .\sample_data\comp.dax .\sample_data\comp_mlist.bin .\sample_data\3d_zfit.xml
- with troubleshooting help from Jeff, got DaoSTORM working on Monet. Built a mobile, pre-compiled version of DaoSTORM to run on any 64-bit windows computer
- RunDotFinder GPUmultifit with Nmax = 3 and 5000 frame batch size ran on H:\2013-01-23_BXC\STORM\ data and finished in 4.213 hours. Single movie analyzed in 16 minutes, an average of 26 fits per second.
To Do
- Comparison of speed of GPUmultifit to DaoSTORM and InsightM runspeeds. (Requires first changing Tuck over to use the new repo.)
- New plan for integrating extra libraries: dlls go in a common folder (L1_Homotopy and DaoSTORM use most of the same ones, as will any new c code we write. This avoids having to have extra copies of these rather large files in the repo). The startup script handles attaching dependencies and function calls.