Category Archives: Fly Work

fly work

Posted on April 7, 2011 by admin

Flip bcd-3UTR flies into bottle. Flip collection plates, 10:50A, (cact/HbZ and MP09 Hz lines). Sort previous embryo collections, 3x snail in vnd[D38], E(spl) x vnd[D38], E(spl); some already stained see in situs 01/07/10 Flip plates again 1:50, Fix 3:00 P. … Continue reading →

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Friday, 03/25/11

Posted on March 25, 2011 by admin

10:30 A – 9:20 P Making figures for Fly Meeting presentation. Figure ‘pol pausing as a checkpoint’ for ML Found bug in CheckDotUpDown.m while making methods figures. (Causes missing of stacked dots) Suspected this might exist, need to re-run dot-counting. … Continue reading →

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Monday 03/07/11

Posted on March 7, 2011 by admin

10:30 A — 7:00P, 9:00P — 12:00A Collect virgin hb rescues AM Sort hb-rescues: 3 vials of 1×1 w; BAC11/CyO; hb[D]/TM3,Sb,Ser,hb-LacZ (~1:200?) 1 vial of 2×10 w; BAC11-wing-Sp/CyO; hb[D]/TM3,Sb,Ser,hb-LacZ 1 virgin BAC03/CyO; hb[D]/TM3,Sb,Ser,hb-LacZ, no males.  (~1:200?) Finish O/N confocal run on … Continue reading →

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Sunday 02/13/11

Posted on February 13, 2011 by admin

12:45 P – 7:00 P, 9:00P — 12:10 A Finish confocal run (MP10, slide from 2/2/11 in situs) Collect virgins from DB04 cross. Analyzing new MP10 data New fog- collection cage.  fog flies ready to collect virgins and cross to … Continue reading →

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Fly work

Posted on February 7, 2011 by admin

Flip collection cages, 10:30 A water crosses, (male 04; Pr x DGTG are failing/dying).  Other 04s look okay. Flip collection cages, 1:15P

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Understanding bursty Gal4-LacZ expression

Posted on February 1, 2011 by admin

Researching UAS-LacZ line sequences to probe stochastic expression effects. my UAS-LacZII from flybase stock 3955, From Kathy Matthews, 1998 gift of flybase. contains: P{UAS-NZ}20b — SV40 T NLS-lacZ fusion in pUAST (beta-gal is localized to the nucleus). Insertion on chromosome … Continue reading →

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Fly stocks

Posted on January 20, 2011 by admin

Flip all: 2x MG-alpha into new bottles fog virgins into new bottles (for collection after Gordon conference). New cross: fog virgin x hkb:fog2 (orig. stock #M25). water all fly crosses.  Fog rescue crosses still look infertile (not surprising with 1:25 … Continue reading →

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Fly Work

Posted on January 18, 2011 by admin

Flip collection cages, 2x mat-a Gal4 x UAS-twi2, UAS-da, UAS-m8: 10:50A Flip collection cages, 2:00P, Fix embryo, 3:00 P Flip bottle stocks, add foam lids to bottle stocks and two working flats of bottles.

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Fly Work

Posted on January 15, 2011 by admin

Flip collection plates, 12:30 A Print fly labels Water all fly crosses: BAC 03 x Hb[D], BAC 11 x Hb[D], BAC 04 x DBTG, BAC 04 x DBTZ, backup lines 3; HbZ, 11;HbZ.

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Fly Work

Posted on January 14, 2011 by admin

Flip collection cages 10:20 (interupted by post-doc interview stuff) Flip collection cages 3:40 P. #  Flip cages, 6:40, Fix embryos 8:00P.

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