Category Archives: Chromatin

choosing insect cell lines for chromatin STORM

Posted on October 29, 2012 by admin

Info on adhesion. From: http://www.flyrnai.org/DRSC-PRC.html.  Also has protocols for maintenance.  Table shows cell line, growth at RT, recommend dilution when splitting cells. A. Semi-adherent cell lines – will stick to new flask but come loose over time S2* 3-4 days 1:3 – 1:4 … Continue reading →

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Aptamers for chromatin

Posted on October 17, 2012 by admin

histone aptamers H4 (+H3) aptamer (publication) 5′- {Alexa Fluor 647} – {Amino Modifier C6 dT} – GGT GGG GTT CCC GGG AGG GCG GCT ACG GGT TCC GTA ATC AGA TTT GTG T – 3′ Need to improve crosslinking of small … Continue reading →

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observations on Insulator organization

Posted on October 16, 2012 by admin

CTCF sites are TF rich: (en locus) CTCF sites are TF rich ~HOT DNAs?  (though not as strong DNA-hypersensitive regions).  rich ~HOT DNAs?  (though not as strong DNA-hypersensitive regions). Do these insulators co-localize (most insulators HiC/3C together) Coerces 2012 MolCell chromatin domain boundaries … Continue reading →

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Protected: Analysis of En locus k27me3

Posted on October 15, 2012 by admin

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Protected: Pc clustering over time (at SR)

Posted on October 15, 2012 by admin

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Protected: Pc and K27me3 dual labeled first analysis

Posted on October 15, 2012 by admin

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Protected: K27me3 density

Posted on October 14, 2012 by admin

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Friday 10/12/12

Posted on October 12, 2012 by admin

9:45 A – 7:00 P, 8:00P-9:15P Fly work collect virgins: E(spl)[D], sim[D], MTD new bottle cross: MTD virgins x male TRiP Scm shRNA 5 new vials cross E(spl)[D] x sim[D] collect PM virgins E(spl) and sim MTD moved back to … Continue reading →

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Protected: Dynamics of K27me3 at AbdB

Posted on October 12, 2012 by admin

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Protected: polycomb silencing super-resolution images at ANT-C, BX-C and En loci

Posted on October 10, 2012 by admin

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