Info on adhesion. From: http://www.flyrnai.org/DRSC-PRC.html. Also has protocols for maintenance. Table shows cell line, growth at RT, recommend dilution when splitting cells.
A. Semi-adherent cell lines – will stick to new flask but come loose over time
| S2* | 3-4 days | 1:3 – 1:4 |
| S2c | 3-4 days | 1:3 – 1:4 |
| Kc(167) | 3-4 days | 1:3 – 1:4 |
| l(2)mbn | 3-4 days | 1:3 – 1:4 |
| DL1 | 3-4 days | 1:8 (1:5 – 1:10) |
| DL2 | 3-4 days | 1:8 (1:5 – 1:10) |
| S3 | 3-4 days | 1:3 |
| S2R+ | 3-4 days | 1:3 – 1:4 |
| CL8 | 4-5 days | 1:5-1:10 |
| BG2 | 4-5 days | 1:3-1:4 |
| BG6 | 4-5 days | 1:3-1:4 |
How about BG3s? (S2 and BG3s seem the most analyzed modencode lines).
From Joe: BG3s neuronal type, send short projections, adherent but can be detached with vigerous pipetting.
Order fly-lines from Drosophila Genomics Resource Center: https://dgrc.cgb.indiana.edu/cells/store/catalog.html