Monthly Archives: January 2011

Sunday 01/16/11

Posted on January 16, 2011 by admin

1o:00P – 11:30P Flip collection cages (MG2a x UAS-Da, UAS-twi-twi and UAS-m8). Water fly crosses (76 vials). Reviewing lab meeting presentation.

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Saturday, 01/15/11

Posted on January 15, 2011 by admin

11:00A – 3:00P, 4:00P-8:00P Working on lab meeting presentation Fixed bugs with pdf normalization. Extended hb calculations to account for data at multiple cell cycles — assume a single interaction probability must match data at all cell cycles. (11, 12, … Continue reading →

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Fly Work

Posted on January 15, 2011 by admin

Flip collection plates, 12:30 A Print fly labels Water all fly crosses: BAC 03 x Hb[D], BAC 11 x Hb[D], BAC 04 x DBTG, BAC 04 x DBTZ, backup lines 3; HbZ, 11;HbZ.

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high resolution imaging

Posted on January 15, 2011 by admin

More high resolution imaging of mRNA cytoplasmic transcripts. Depth indicated by color. To image: sna-y-full MP05, MP06, and MP10 (snail with and without shadows). To stain: hb-y-full dig, MP01, MP02, MP09

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Friday 01/14/11

Posted on January 14, 2011 by admin

9:30A – 7:20P, 8:30P – 11:50 P Discussion with Oster, should start writing general PD apps as plan B. Visiting post-doc candidate (Marco from Zurich, translation regulation). Confocal : more images of hb and M-alpha Working on dot-counting and dot … Continue reading →

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Fly Work

Posted on January 14, 2011 by admin

Flip collection cages 10:20 (interupted by post-doc interview stuff) Flip collection cages 3:40 P. #  Flip cages, 6:40, Fix embryos 8:00P.

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Cell-cell variation in cytoplasmic mRNA

Posted on January 13, 2011 by admin

Using new Zeiss scope to quantify cell-cell variation in cytoplasmic mRNA. Note very few dots bleed through channels — most dots are red, blue, and green, rather than yellow, or cyan. High variation in Mg-alpha II LacZ expression, pretty low … Continue reading →

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Thursday 01/13/10

Posted on January 13, 2011 by admin

10:00 A – 7:00 P, 8:20P – 11:35 P Biophysics quals advice meeting. Fly work and embryo fixin’, see below Confocal (with new Zeiss 700): high res Mat-alpha II x LacZ, compare to uniform expression of hb.   Working on … Continue reading →

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Fly work

Posted on January 13, 2011 by admin

2x mat-gal4-alpha x UAS-da[M2], twi2, and Espl/m8 Flip collection plates: 1:48 Remove collection plates for aging 4:48 Fix embryos: 5:48 (1-4 hr collection). Collect w; Sp/CyO; Hb[D]/GFP Collect DBTZ and DBTG males and virgins Cross MP04 x DBTZ (2 vials each … Continue reading →

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Wednesday 01/12/11

Posted on January 12, 2011 by admin

10:20 A – 7:00 P, 8:30P – 11:50 P Ready to cross MP04s into DBTG and DBTZ after tomorrows v-collection. Working on slides and figures and for hb enhancer modeling (figures take forever). Espl, twi-twi, and da driven by 2x … Continue reading →

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