Daily Archives: January 13, 2011

Cell-cell variation in cytoplasmic mRNA

Posted on January 13, 2011 by admin

Using new Zeiss scope to quantify cell-cell variation in cytoplasmic mRNA. Note very few dots bleed through channels — most dots are red, blue, and green, rather than yellow, or cyan. High variation in Mg-alpha II LacZ expression, pretty low … Continue reading →

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Thursday 01/13/10

Posted on January 13, 2011 by admin

10:00 A – 7:00 P, 8:20P – 11:35 P Biophysics quals advice meeting. Fly work and embryo fixin’, see below Confocal (with new Zeiss 700): high res Mat-alpha II x LacZ, compare to uniform expression of hb.   Working on … Continue reading →

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Fly work

Posted on January 13, 2011 by admin

2x mat-gal4-alpha x UAS-da[M2], twi2, and Espl/m8 Flip collection plates: 1:48 Remove collection plates for aging 4:48 Fix embryos: 5:48 (1-4 hr collection). Collect w; Sp/CyO; Hb[D]/GFP Collect DBTZ and DBTG males and virgins Cross MP04 x DBTZ (2 vials each … Continue reading →

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