Monthly Archives: March 2011

Monday 03/07/11

Posted on March 7, 2011 by admin

10:30 A — 7:00P, 9:00P — 12:00A Collect virgin hb rescues AM Sort hb-rescues: 3 vials of 1×1 w; BAC11/CyO; hb[D]/TM3,Sb,Ser,hb-LacZ (~1:200?) 1 vial of 2×10 w; BAC11-wing-Sp/CyO; hb[D]/TM3,Sb,Ser,hb-LacZ 1 virgin BAC03/CyO; hb[D]/TM3,Sb,Ser,hb-LacZ, no males.  (~1:200?) Finish O/N confocal run on … Continue reading →

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Sunday 03/06/11

Posted on March 6, 2011 by admin

11:00A – 3:00P,5:00P – 7:00P, 9:00P – 11:00P Finish flipping fly stocks. Collect fly lines for James (have 16 requested lines.  Still need MP10 and snaD; sna2.8 line) Start new fly-stock list: ANB_PermStocks (for lab archive). Matlab code for converting … Continue reading →

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Image Processing coding

Posted on March 6, 2011 by admin

Matlab BUG FOUND !! if given an input of type single or double im2bw thresholds at x>T = 1 and x <T  =0, regardless of the range of the input variable x. T is however restricted to be between 0 and 1. … Continue reading →

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Saturday 03/05/11

Posted on March 5, 2011 by admin

12:00 P — 7:00P, 8:40P — 1:30A Finish confocal 1xMGa_LacZ. Major image processing recoding (see post). Collect virgin BAC rescues

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Friday 03/04/11

Posted on March 4, 2011 by admin

8:30 A – 9:15, 10:00A-11:50P Finish O/N confocal run on 2xMGa LacZ and snail. Observations: in Tony’s paper, most snail primary not repressed in posterior pole (AS proposes that snail shadow is responsible). Improving dot finding (see post) Jacques’ Lab … Continue reading →

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Improving dot filtering

Posted on March 4, 2011 by admin

with minimum threshold, (min_int = .01), lots more exogenous dots with poorer gaussian fit and low intensity.  Note: intensities intentionally rescaled, saturating bright dots, in order to make visible the low intensity pixels.     $latex \alpha_E$ = 1; sigmaE = … Continue reading →

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Thursday 03/03/11

Posted on March 3, 2011 by admin

10:00 A — 7:00P, 7:45P — 12:00A Collect virgin BAC 3/11 x Hb[D] Working with Jen to fix imviewer (reqiures fxnproject, fxnclean_v2, fast_rotate.cpp [mex] and more).  Need to create a more functional version on Im repo. analyzing hb imviewer_lsm.m and … Continue reading →

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Improving mRNA dot finding

Posted on March 3, 2011 by admin

3D visualization with nuclei:

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Wednesday 03/02/11

Posted on March 2, 2011 by admin

9:40 A – 4:00 P, 8:30P – 10:20 P Cleaning up data folder on Zeiss-700 computer. Fix minor issues in Supplement for Weiner Paper, return to George. Re-shorten abstract for Markov paper, send to Peter and Steve for edit/approval.  Resubmitted. … Continue reading →

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Tuesday 03/01/11

Posted on March 1, 2011 by admin

10:00 A – 7:10 P , 10:45 P — 11:50 P Complete confocal run on MP09 22C hets (stained 02/17/11).  Completed 10 embryos, all before posterior expression activates. Start flipping fly stocks Markov paper resubmitted Convert Weiner paper supplement to … Continue reading →

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