Daily Archives: March 15, 2011

Analyzing effect of increasing Gal4

Posted on March 15, 2011 by admin

Images with high variability have high overall average expression rate in MGa2x, images with more uniform expression have lower levels.  The reverse is true for MGa1x. MGa1x          

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Tuesday 03/15/11

Posted on March 15, 2011 by admin

9:50 A — 4:00P, 8:00P — 11:00P Finish confocal run MP09_22C Run Unsupervised_DotFinding on MGa2x and 1x data.  Changed Gaussian filter to sigma_E,I = 2.5,3 instead of 3.5,4 (make better classification). Discussion with ML on gap shadows (finally) Sort all … Continue reading →

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Image processing

Posted on March 15, 2011 by admin

Working on analysis for MGa1x vs 2x. Shown left, mRNA densities for MGa2x.  Internal control for MGa2x achieved by measuring expression.     With Mga 1x, compare ush(?) intronic probe as a measure of background.  Only 0-5 dots found per … Continue reading →

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