Monthly Archives: June 2012

Thursday 06/07/12

Posted on June 7, 2012 by admin

10:20 A – 6:50P, 8:30P – 11:20P in situs day 3 with KE. 17 slides mounted to test tomorrow [slideshow 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 600 300 sheet=7] Prep STORM slide of Su(Z)12-750, K27-647 STORM imaging of Pc-GFP slide Beads arrays in 3 focal planes … Continue reading →

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Wednesday 06/06/12

Posted on June 6, 2012 by admin

10:30 A – 1:00P, 4:00P-7:00 P troubleshooting 3d warping (see post) Final problem looks like artifact from trying to get 3D fitting data from 2D planes of beads.  Try to shoot more beads tomorrow. in situs day 2 with KE … Continue reading →

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chromatic warp issues

Posted on June 6, 2012 by admin

warped image blurs out certain channels.  here 561 gets stretched along x. In the K27/K4 dataset 750 is dramatically stretched along x-y in a correlated fashion. Distortions get worse using higher order polynomial (e.g. 4th order).   Distortions are no … Continue reading →

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Tuesday 06/05/12

Posted on June 5, 2012 by admin

10:00A – 8:00P in situs day 1 with KE: new large cage of Pc-GFP and Yw Flip 3x Mat-Gal4 into new bottles.  Moved to 25C. troubleshooting chromatic warp issues in new vector storm code.

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Monday 06/04/12

Posted on June 4, 2012 by admin

10:00 A-12:00P, 5:30P-7:30P, 9:30P-10:30P Brian group meeting Katherine Ebright starting summer program in lab. working on vector image analysis for K27me3, K4me3

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