Monthly Archives: July 2012

Tuesday 07/31/12

Posted on July 31, 2012 by admin

11:00 A – 7:40P, 8:20P-11:00P (volleyball practice 9A-11A) Flip fly cages KE fix embryos 2-6 hrs old (Pc-GFP and YW) Reorganizing text for kinetics paper: homogeneity, dynamics and kinetics, dosage compensation. Adapt figure_sna_dynamics to show spatial profiles over time. STORM centromere … Continue reading →

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Monday 07/30/12

Posted on July 30, 2012 by admin

9:50 A – 5:00 P, 9:30P – 10:45p Jiang lab meeting difficulty connecting to notebook 710 confocal all sessions canceled, new 780 being installed this week.  Requested training on ELYRA which also has a 710 (and maybe a programable stage?) … Continue reading →

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Protected: first look at promoter mutants

Posted on July 30, 2012 by admin

There is no excerpt because this is a protected post.

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Sunday 07/29/12

Posted on July 29, 2012 by admin

9:30 A- 10:00A, 11:30A-6:30P Rerunning multi-label counting controls and probe competition experiments. control 1 is tiled with multiple red and green probes.  control 2 competes two short, contiguous probes for the same sequence.  Since these rarely manage to give overlapping … Continue reading →

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Saturday 07/28/12

Posted on July 28, 2012 by admin

10:30 A -2:00P, 4:30P-7:30P, 9:50P-11:00P Finish O/N confocal of m197.  Imaging plane too high, lower sections of most embryos not fully imaged.  Set up rescan. ~10:30A. working on parsing genomic data to test changes in H3K27me3 occupancy in A8 using … Continue reading →

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Friday 07/27/12

Posted on July 27, 2012 by admin

9:40 A – 7:40, 9:00P – 12:30A Finished confocal run, 8.5 embryos finished at speed 5 by 10A (run started at 10P). Previous user canceled 710 time (again).  Need to check before starting or stopping a run. Over-etched first K27 … Continue reading →

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Protected: Effects of bistability

Posted on July 27, 2012 by admin

There is no excerpt because this is a protected post.

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Thursday 07/26/12

Posted on July 26, 2012 by admin

10:20 A New fly cages of Pc-GFP and yw.  Pc-GFP not growing well, should have ~10 bottles/cage.  4 would be sufficient for yw cage. Crossed virgin 3x mat Gal4 x Pc-shRNA2 in vial test viability. new bottle of 3x mat … Continue reading →

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Wednesday 02/25/12

Posted on July 25, 2012 by admin

10:00 A – 7:30P, 8:50P – 12:15 A checking sna-y counts.  Rerunning analysis scripts on MP10 and MP05 data seems to me MP05 have more counts than MP10 in both 2012-02-17 data and 2012-06 and 2012-05 datasets.   Not consistent … Continue reading →

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Tuesday 07/24/12

Posted on July 24, 2012 by admin

10:00A – 12:30A Revising snail kinetics paper for NSMB Revised Supp Fig 4, revised kinetics paragraph for multicopy.  Recomputed approach to saturation regime controlled by intrinsic kinetics of Pol II. confocal imaging of sample 1 from Mounia KE repeating PCR for new … Continue reading →

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