Monthly Archives: June 2012

Saturday 06/30/12

Posted on June 30, 2012 by admin

Flip fly cages.  Old Pc-GFP dying –> freeze. Need to characterize loss of snail mRNA in snail nulls better.  ~early cc14 levels look like even half peak cc13 + degradation: ~85 transcripts per cell (highs just over 100).  late cc14 … Continue reading →

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Thursday 06/28/12

Posted on June 28, 2012 by admin

10:15 A – 7:10 P antibodies to label: dk a-shp Cy3B.   dk a-m Atto488  (with new R&D antibodies: dk a-m 750, dk a-rb 750 make new fly plates washing out tertiary antibodies on in situs hot washes for PcG … Continue reading →

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Wednesday 06/27/12

Posted on June 27, 2012 by admin

10:10 A – 11:00P in situs day 3 washing out and post-fixing Pc labeled primaries write to Nicole Follmer about antibodies lit review: piRNA in C elegans multigenerational epigenetic memory.  LacR facilitated diffusion.  DNA sequence dependent compartmentalization and silencing an … Continue reading →

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Tuesday 06/26/12

Posted on June 26, 2012 by admin

10:30 A -5:00P, 9:00P – 11:30P in situs day 2 short fix 2-14 hour yw and Pc embryos. rehydrate and stain 2-14 hour short fix embryos with Su(Z)12.  Pc-GFP embryos also stained with ch a-GFP. vball lost. Synthesized Bac 1 … Continue reading →

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Monday 06/25/12

Posted on June 25, 2012 by admin

9:45 A – 7:00P, 9:30P – 11:45P Need new collection cages FISH + K27 on O/N embryos day 1 Flipped 3x Mat Gal4 cages. Running Z-calibration analysis on Suz647 data (with Quadview) Collecting new z-cal bead data in 512 and … Continue reading →

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Sunday 06/24/12

Posted on June 24, 2012 by admin

Feedback from ML on dynamics paper.  Need to chose journal.  Homogeniety section could use some tightening.  

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Saturday 06/23/12

Posted on June 23, 2012 by admin

11:00 A -7:00P Sunny’s 50th: commerative conference Gene regulation session. Working on snail manuscript analyzing MT data. Overwrote Bead1 data with Bead2 acquisitions… Instability in z-calibration: uncertainty bounds of zr and other parameters increase with iterations instead of decrease! Load … Continue reading →

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Friday 06/22/12

Posted on June 23, 2012 by admin

10:30 A – 5:30, 8:30-11:30P Make new bead slide with 40 nm 540 beads and 100 nm IR beads. Finish O/N STORM run and shoot beads. Fixed issue with 3D bead mapping not mapping IR and Vis 647 channels together. … Continue reading →

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Thursday 06/21/12

Posted on June 21, 2012 by admin

2:00P — 8:30P Calibrating new bead imaging: 1:20 then 1:500 540 beads + 1:500 Q-dots-705.  Too dense. Dilute a further 1:500.  Q dots good density.  Blinky, excited best by 405 or 488.  Look good in 647, not really visible in 750 … Continue reading →

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Wednesday 06/20/12

Posted on June 20, 2012 by admin

10:50 A – 6:30P ordered 0.04 um Fluospheres (540 + 715).  large fluospheres give unusable 3D maps No 715 Fluospheres, ordered 20-nm 705 quantum dots Fixed error in Z-cal convergence.  Modified convergence measure to use both x and y data. … Continue reading →

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