Monthly Archives: May 2012

Wednesday 05/30/12

Posted on May 30, 2012 by admin

12:00 P -8:00P clean and coat 2 racks of small coverslips confocal analysis of embedded oligo-paint sample.  Embryos not detectable. sectioning of Su(Z)12-750 K27me3-647 + Dm0-750,647 working on new vector version STORM analysis code.

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Tuesday 05/29/12

Posted on May 29, 2012 by admin

1:00 P – 4:00P, 9:00P-12:00A running insight analysis on K4 K27 data  (11:00A) using only single iteration for computation of z-calibration curve. compare structure of K4, K27me3 and K27ac chromatin (?) troubleshooting issue with non-convergence of z-calibration bead data by … Continue reading →

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Protected: snail papers

Posted on May 29, 2012 by admin

There is no excerpt because this is a protected post.

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Protected: snail dose effects

Posted on May 28, 2012 by admin

There is no excerpt because this is a protected post.

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Monday 05/28/12

Posted on May 28, 2012 by admin

10:40 A – 7:00P, 8:45P – 11:45 P Flip fly bottles: Pc-GFP.  Now growing at 25C Flip into single bottles for bottle stock: Pc-shRNA1 and 2, Psc-shRNA1,  Bcd-GFP, eve-GFP, en-GFP.  Pc-shRNA lines strongly female gender biased. Primary stocks at 18C look … Continue reading →

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Drosophila Systems Biology, ESF Poland

Posted on May 25, 2012 by admin
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ESF Poland conference notes

Posted on May 25, 2012 by admin

  unedited notes taking during talks. David Arnosti Thermodynamic and really dynamics modeling of transcription. how transcription factors talk to eachother on enhancers short-range and long-range repressors – global deacytilation (focus on short). Conservation of eve stripe enhancers despite limited … Continue reading →

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Monday 05/14/12

Posted on May 14, 2012 by admin

10:00A – 8:30P, 9:30-10:30P Eileen Group meeting invitation to SDB to chair postdoc session and speak in regulatory mechanisms section NuGru presentation: Swr1 structural analysis (inserts H2AZ flanking nucleosome depleted regions), for example to protect promoter. Finished 3D bead warping … Continue reading →

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Saturday 05/12/12

Posted on May 12, 2012 by admin

11:30 A – 5:30P Finish DR acceptance statement / bio etc. Literature review: Shandilya transcription review, evidence for transcription factories: Xu 2008 (minichromosomes), Osborne 2004 (DNA / RNA FISH comparison) working on 3D vector bead alignment code

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Friday 05/11/12

Posted on May 11, 2012 by admin

10:55 A -5:15P photos for DRF freeze and clean out fly cages review 3D bead alignment with Sang-Hee Reviewing parameter files.  My default  max displacement don’t match other parameters – might explain low intensities? reset: max_displacement = 1 (160 nm), … Continue reading →

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