Monthly Archives: April 2012

Monday 04/30/12

Posted on April 30, 2012 by admin

9:45 A – 8:15P, Steven group meeting presentation sequencing results: AbdB-1A: sense strand.  Use SP6 for antisense probe AbdB-1B: sense strand.  Use SP6 AbdB-3A: sense strand. poor quality read AbdB-3B: antisense strand. Use T7 Dfd-2A: antisense strand: T7 Dfd-2B: antisense … Continue reading →

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Sunday 04/29/2012

Posted on April 29, 2012 by admin

4:00 P -9:30 P STORM analysis of Pc-GFP: Insight froze on 647 channel at ~1.5 million molecules : error program tried to terminate incorrectly. min height on all parameter files somehow got stuck at 750 levels (30).  Reset manually.  stem … Continue reading →

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Saturday 04/28/12

Posted on April 28, 2012 by admin

11:00 A – 11:45 P Confocal imaging of Pc-GFP and Pc Reading up on DNA FISH from Bantignies protocol.  10 min denature probe at 95C.  15 min, denature embryos in prehybe at 80C.  Combine hot. wash 1hr at 37 with … Continue reading →

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Friday 04/27/12

Posted on April 27, 2012 by admin

10:20A – 5:10P, 9:00P – 11:00P Etch hb-y(i), Bcd sample STORM hb-y(i), Bcd sample remember to turn 642 laser power back up! miniprep colonies: AbdB-1A,B AbdB-3A,B, Dfd-2A,B, 5E-A,B.  all over 100 ng/uL. send 500 ng for sequencing to GeneWiz. move ligation … Continue reading →

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Thursday 05/26/12

Posted on April 26, 2012 by admin

10:00 A – 11:45 P Check colonies from Bx-C round 1 cloning: all plates look good, no lawns. Gel-extract linearized Taf, Tub Gel-extract newly cloned Bx-C probes Antibody synthesis: antibodies 61-69 Flip collection cages: 10:15 A Washing out primary antibody … Continue reading →

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Wednesday 04/25/12

Posted on April 25, 2012 by admin

9:45 A – 8:30 P O/N hybridization — should do in PCR block.  Water evaporates from 37C block, not good contact with PCR tube.  Refilled water, allow another hour of hybrdization before washes. Sequencing results:  A1 = Taf1 antisense (sequence with m13F … Continue reading →

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Tuesday 04/24/12

Posted on April 24, 2012 by admin

10:20 A – 5:15P, 8:00P-9:00P, 10:20P-12:30A Flip Fly cages, 10:30A Trimming oligo-STORM sample new bottles of Yw (4), MP09 (6) and Pc-GFP (4) from collection cages Mendeley advisor’s meeting STORM run Pc-GFP Fix Pc-GFP embryos –> prepped for oligo-STORM Fix … Continue reading →

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Monday 04/23/12

Posted on April 23, 2012 by admin

9:45 A – 11:30P Lab meeting presentation John Wu’s thesis defense Consolidated disk drives: new data on external drives Alistair4 and Alistair5 backed up to empty gaps slots on Alistair2 and 3. Flip fly cages – 2:30P Analyze Psc localization, … Continue reading →

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Sunday 04/22/12

Posted on April 22, 2012 by admin

10:30 A -5:00P Fly work Flipped all Gal4-Bcd3’UTR lines into new bottles (8).  Moved to 25C.  Flip again in 2-3 days, start virgin collection in 10 days. New cages of MP09, yw, and Pc-GFP. Disposed all bottles of MP09 and … Continue reading →

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Saturday 04/21/12

Posted on April 21, 2012 by admin

10:45 A – 7:45 P, 10:30P – 11:30 P Working on lab meeting presentation slide organization new analysis of Psc, H3K27, H3 localization differences HP1a doesn’t haven’t correct conventional level nuclear localization Samples to cut and image 4H  — K27me3-750, … Continue reading →

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