11:00 A – 11:45 P
- Confocal imaging of Pc-GFP and Pc
- Reading up on DNA FISH from Bantignies protocol. 10 min denature probe at 95C. 15 min, denature embryos in prehybe at 80C. Combine hot.
- wash 1hr at 37 with post hybe, (4 washes), 1 hr at RT with post-hybe
- Attempted N-STORM of oligo-labeled cells. Lock for perfect focus is much too far away from focal plane of cells to work.
- Request 3X Gal4 line from Perrimon
- Writing chromatic correction script for Zeiss from beads.
- Revised automated z-calibration to test for convergence of z-parameter set (based on first parameter only. Could extend to all parameters).
- Analyzing bead data from Pc-GFP STORM imaging using revised z-cal script.
- Analyzing STORM data from Pc-GFP STORM imaging.
- Transform competent cells with ligations: Fab8, 7E, 2E, AbdB2,