Monthly Archives: March 2012

Saturday 03/31/12

design primers for AbdB regions Confocal imaging of MP08 slides from 02/06 in situs: focus on getting more cycle 13 embryos.

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Friday 03/30/12

9:20A — 8:00 P Shuttle to Med campus to see Xiaochen talk (White lab student interviewing for Perrimon / Bulyk labs) Very clean CTCF data.  Should try this antibody in STORM Finalize ACS application.  Submitted paper copy and FEDEX’d. Still … Continue reading

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Thursday 03/29/12

10:15 A – 8:00P Working on snail dynamics paper. Developing new introduction. new script to change Fig 6 to color code nuclei by mRNA count class rather than direct mRNA levels — easier to interpret with nuclear channel added in.

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Wednesday 03/28/12

10:15 A – 8:00P Analyzing Psc and Pho data with new alignment routine (summary results still need to add images to lab notebook, see protected post) Working on snail manuscript    

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Tuesday 03/27/12

11:00A – 8:00P, Coding Accelerating integrated 4 color chromatic, drift correction into code to convert molecule list to corrected tif images. Notes from STORM presentation STORM2 power report, At 40 x 40 um at 60 Hz: 20 mW 750 at … Continue reading

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Monday 03/26/12

10:00 A – 7:30P, 9;30P-11:00P Lab Cleaning List  of tasks [as reference for next time] Gel Bench (Upstairs) pH Bench (Upstairs and Downstairs) miniprep Bench (Upstairs) weigh station (Upstairs and Downstairs) Clean Fume hood (Upstairs and Downstairs) Clean cell-culture hood (Upstairs and … Continue reading

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Saturday 03/24/12

10:15 A Assemble draft protocol of embryo multiplex RNA-Protein STORM send to Stacie at Stowers. 5 mL of culture in probably too much to get good aeration in small round-bottom tubes.  Try 2 mL next time.  use Zhuang shaker, it … Continue reading

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Protected: DNA-Oligo STORM try 1

There is no excerpt because this is a protected post.

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Friday 03/23/12

10:45 A – 12:30 A O/N confocal 10 um is a little too much buffer between first and last, several embryos didn’t make it all the way through.  Try again tonight. Set up new O/N confocal run 6 um difference … Continue reading

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Thursday 03/22/12

9:45 A -12:20 A O/N confocal run failed horribly.  Despite warm-up, quick reset of positions, and quick calibration run, devastating z change of focus occurred during run. Rinsing out primary antibodies GPU multi-fit code still running slow (70 min+ per channel per … Continue reading

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